Exposure to electronic cigarettes impairs pulmonary anti-bacterial and anti-viral defenses in a mouse model.
Identifieur interne : 000D41 ( Main/Exploration ); précédent : 000D40; suivant : 000D42Exposure to electronic cigarettes impairs pulmonary anti-bacterial and anti-viral defenses in a mouse model.
Auteurs : Thomas E. Sussan [États-Unis] ; Sachin Gajghate [États-Unis] ; Rajesh K. Thimmulappa [États-Unis] ; Jinfang Ma [États-Unis] ; Jung-Hyun Kim [États-Unis] ; Kuladeep Sudini [États-Unis] ; Nicola Consolini [États-Unis] ; Stephania A. Cormier [États-Unis] ; Slawo Lomnicki [États-Unis] ; Farhana Hasan [États-Unis] ; Andrew Pekosz [États-Unis] ; Shyam Biswal [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Charge virale (), Mâle, Nicotine (), Nicotine (effets indésirables), Phagocytose (), Poumon (), Poumon (immunologie), Poumon (microbiologie), Poumon (virologie), Radicaux libres (analyse), Souris, Souris de lignée C57BL, Sous-type H1N1 du virus de la grippe A (physiologie), Streptococcus pneumoniae (physiologie), Stress oxydatif (), Tabagisme (effets indésirables), Volatilisation.
- MESH :
- analyse : Radicaux libres.
- effets indésirables : Nicotine, Tabagisme.
- immunologie : Poumon.
- microbiologie : Poumon.
- physiologie : Sous-type H1N1 du virus de la grippe A, Streptococcus pneumoniae.
- virologie : Poumon.
- Animaux, Charge virale, Mâle, Nicotine, Phagocytose, Poumon, Souris, Souris de lignée C57BL, Stress oxydatif, Volatilisation.
English descriptors
- KwdEn :
- Animals, Free Radicals (analysis), Influenza A Virus, H1N1 Subtype (physiology), Lung (drug effects), Lung (immunology), Lung (microbiology), Lung (virology), Male, Mice, Mice, Inbred C57BL, Nicotine (adverse effects), Nicotine (chemistry), Oxidative Stress (drug effects), Phagocytosis (drug effects), Smoking (adverse effects), Streptococcus pneumoniae (physiology), Viral Load (drug effects), Volatilization.
- MESH :
- chemical , adverse effects : Nicotine.
- chemical , analysis : Free Radicals.
- chemical , chemistry : Nicotine.
- adverse effects : Smoking.
- drug effects : Lung, Oxidative Stress, Phagocytosis, Viral Load.
- immunology : Lung.
- microbiology : Lung.
- physiology : Influenza A Virus, H1N1 Subtype, Streptococcus pneumoniae.
- virology : Lung.
- Animals, Male, Mice, Mice, Inbred C57BL, Volatilization.
Abstract
Electronic cigarettes (E-cigs) have experienced sharp increases in popularity over the past five years due to many factors, including aggressive marketing, increased restrictions on conventional cigarettes, and a perception that E-cigs are healthy alternatives to cigarettes. Despite this perception, studies on health effects in humans are extremely limited and in vivo animal models have not been generated. Presently, we determined that E-cig vapor contains 7 x 10(11) free radicals per puff. To determine whether E-cig exposure impacts pulmonary responses in mice, we developed an inhalation chamber for E-cig exposure. Mice that were exposed to E-cig vapor contained serum cotinine concentrations that are comparable to human E-cig users. E-cig exposure for 2 weeks produced a significant increase in oxidative stress and moderate macrophage-mediated inflammation. Since, COPD patients are susceptible to bacterial and viral infections, we tested effects of E-cigs on immune response. Mice that were exposed to E-cig vapor showed significantly impaired pulmonary bacterial clearance, compared to air-exposed mice, following an intranasal infection with Streptococcus pneumonia. This defective bacterial clearance was partially due to reduced phagocytosis by alveolar macrophages from E-cig exposed mice. In response to Influenza A virus infection, E-cig exposed mice displayed increased lung viral titers and enhanced virus-induced illness and mortality. In summary, this study reports a murine model of E-cig exposure and demonstrates that E-cig exposure elicits impaired pulmonary anti-microbial defenses. Hence, E-cig exposure as an alternative to cigarette smoking must be rigorously tested in users for their effects on immune response and susceptibility to bacterial and viral infections.
DOI: 10.1371/journal.pone.0116861
PubMed: 25651083
Affiliations:
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Sussan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Gajghate, Sachin" sort="Gajghate, Sachin" uniqKey="Gajghate S" first="Sachin" last="Gajghate">Sachin Gajghate</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Thimmulappa, Rajesh K" sort="Thimmulappa, Rajesh K" uniqKey="Thimmulappa R" first="Rajesh K" last="Thimmulappa">Rajesh K. Thimmulappa</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Ma, Jinfang" sort="Ma, Jinfang" uniqKey="Ma J" first="Jinfang" last="Ma">Jinfang Ma</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Kim, Jung Hyun" sort="Kim, Jung Hyun" uniqKey="Kim J" first="Jung-Hyun" last="Kim">Jung-Hyun Kim</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Sudini, Kuladeep" sort="Sudini, Kuladeep" uniqKey="Sudini K" first="Kuladeep" last="Sudini">Kuladeep Sudini</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Consolini, Nicola" sort="Consolini, Nicola" uniqKey="Consolini N" first="Nicola" last="Consolini">Nicola Consolini</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Cormier, Stephania A" sort="Cormier, Stephania A" uniqKey="Cormier S" first="Stephania A" last="Cormier">Stephania A. Cormier</name><affiliation wicri:level="2"><nlm:affiliation>Children's Research Foundation Institute, University of Tennessee Health Science Center, 50 N. Dunlap, Memphis, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Children's Research Foundation Institute, University of Tennessee Health Science Center, 50 N. Dunlap, Memphis, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Lomnicki, Slawo" sort="Lomnicki, Slawo" uniqKey="Lomnicki S" first="Slawo" last="Lomnicki">Slawo Lomnicki</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Sciences, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Hasan, Farhana" sort="Hasan, Farhana" uniqKey="Hasan F" first="Farhana" last="Hasan">Farhana Hasan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Pekosz, Andrew" sort="Pekosz, Andrew" uniqKey="Pekosz A" first="Andrew" last="Pekosz">Andrew Pekosz</name><affiliation wicri:level="2"><nlm:affiliation>W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Biswal, Shyam" sort="Biswal, Shyam" uniqKey="Biswal S" first="Shyam" last="Biswal">Shyam Biswal</name><affiliation wicri:level="2"><nlm:affiliation>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Free Radicals (analysis)</term><term>Influenza A Virus, H1N1 Subtype (physiology)</term><term>Lung (drug effects)</term><term>Lung (immunology)</term><term>Lung (microbiology)</term><term>Lung (virology)</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Nicotine (adverse effects)</term><term>Nicotine (chemistry)</term><term>Oxidative Stress (drug effects)</term><term>Phagocytosis (drug effects)</term><term>Smoking (adverse effects)</term><term>Streptococcus pneumoniae (physiology)</term><term>Viral Load (drug effects)</term><term>Volatilization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Charge virale ()</term><term>Mâle</term><term>Nicotine ()</term><term>Nicotine (effets indésirables)</term><term>Phagocytose ()</term><term>Poumon ()</term><term>Poumon (immunologie)</term><term>Poumon (microbiologie)</term><term>Poumon (virologie)</term><term>Radicaux libres (analyse)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Sous-type H1N1 du virus de la grippe A (physiologie)</term><term>Streptococcus pneumoniae (physiologie)</term><term>Stress oxydatif ()</term><term>Tabagisme (effets indésirables)</term><term>Volatilisation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Nicotine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Free Radicals</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nicotine</term></keywords><keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Smoking</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Radicaux libres</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lung</term><term>Oxidative Stress</term><term>Phagocytosis</term><term>Viral Load</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Nicotine</term><term>Tabagisme</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term><term>Streptococcus pneumoniae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Streptococcus pneumoniae</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Volatilization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Charge virale</term><term>Mâle</term><term>Nicotine</term><term>Phagocytose</term><term>Poumon</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Stress oxydatif</term><term>Volatilisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Electronic cigarettes (E-cigs) have experienced sharp increases in popularity over the past five years due to many factors, including aggressive marketing, increased restrictions on conventional cigarettes, and a perception that E-cigs are healthy alternatives to cigarettes. Despite this perception, studies on health effects in humans are extremely limited and in vivo animal models have not been generated. Presently, we determined that E-cig vapor contains 7 x 10(11) free radicals per puff. To determine whether E-cig exposure impacts pulmonary responses in mice, we developed an inhalation chamber for E-cig exposure. Mice that were exposed to E-cig vapor contained serum cotinine concentrations that are comparable to human E-cig users. E-cig exposure for 2 weeks produced a significant increase in oxidative stress and moderate macrophage-mediated inflammation. Since, COPD patients are susceptible to bacterial and viral infections, we tested effects of E-cigs on immune response. Mice that were exposed to E-cig vapor showed significantly impaired pulmonary bacterial clearance, compared to air-exposed mice, following an intranasal infection with Streptococcus pneumonia. This defective bacterial clearance was partially due to reduced phagocytosis by alveolar macrophages from E-cig exposed mice. In response to Influenza A virus infection, E-cig exposed mice displayed increased lung viral titers and enhanced virus-induced illness and mortality. In summary, this study reports a murine model of E-cig exposure and demonstrates that E-cig exposure elicits impaired pulmonary anti-microbial defenses. Hence, E-cig exposure as an alternative to cigarette smoking must be rigorously tested in users for their effects on immune response and susceptibility to bacterial and viral infections.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Louisiane</li><li>Maryland</li><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Sussan, Thomas E" sort="Sussan, Thomas E" uniqKey="Sussan T" first="Thomas E" last="Sussan">Thomas E. Sussan</name></region><name sortKey="Biswal, Shyam" sort="Biswal, Shyam" uniqKey="Biswal S" first="Shyam" last="Biswal">Shyam Biswal</name><name sortKey="Consolini, Nicola" sort="Consolini, Nicola" uniqKey="Consolini N" first="Nicola" last="Consolini">Nicola Consolini</name><name sortKey="Cormier, Stephania A" sort="Cormier, Stephania A" uniqKey="Cormier S" first="Stephania A" last="Cormier">Stephania A. Cormier</name><name sortKey="Gajghate, Sachin" sort="Gajghate, Sachin" uniqKey="Gajghate S" first="Sachin" last="Gajghate">Sachin Gajghate</name><name sortKey="Hasan, Farhana" sort="Hasan, Farhana" uniqKey="Hasan F" first="Farhana" last="Hasan">Farhana Hasan</name><name sortKey="Kim, Jung Hyun" sort="Kim, Jung Hyun" uniqKey="Kim J" first="Jung-Hyun" last="Kim">Jung-Hyun Kim</name><name sortKey="Lomnicki, Slawo" sort="Lomnicki, Slawo" uniqKey="Lomnicki S" first="Slawo" last="Lomnicki">Slawo Lomnicki</name><name sortKey="Ma, Jinfang" sort="Ma, Jinfang" uniqKey="Ma J" first="Jinfang" last="Ma">Jinfang Ma</name><name sortKey="Pekosz, Andrew" sort="Pekosz, Andrew" uniqKey="Pekosz A" first="Andrew" last="Pekosz">Andrew Pekosz</name><name sortKey="Sudini, Kuladeep" sort="Sudini, Kuladeep" uniqKey="Sudini K" first="Kuladeep" last="Sudini">Kuladeep Sudini</name><name sortKey="Thimmulappa, Rajesh K" sort="Thimmulappa, Rajesh K" uniqKey="Thimmulappa R" first="Rajesh K" last="Thimmulappa">Rajesh K. Thimmulappa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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